They are among 16 internationals from the men’s and women’s squads to play in a mini tournament on a virtual pitch featuring the video game.
It is part of the #FootballsStayingHome initiative and will get underway on Friday on social media and will also feature esports commentators Brandon Smith and Richard Buckley.
The cast list also features Lucy Bronze, Jordan Nobbs and Ellie Roebuck, Mason Mount, Callum Wilson, James Maddison, Callum Hudson-Odoi and Tammy Abraham as well as Reiss Nelson, Todd Cantwell, Max Aarons, Eddie Nketiah and Ryan Sessegnon.
It will also highlight Government advice to stay home, protect the NHS and save lives, the tournament will highlight the National Emergencies Trust.
The trio are used to facing off against each other on international duty, and recently Rashford even made clear his hopes that Sancho would join him at Man Utd.
Borussia Dortmund are resigned to losing their star winger this summer, with United at the front of the queue to sign him.
The German club have slapped a £120m price tag on the 20-year-old, but Rashford remains hopeful that will not put off his employers.
Asked about the prospect of Sancho joining him at Old Trafford, Rashford told Bleacher Report: “It would be good. Sancho is a great player. He’s like the new generation player.
“It’s definitely exciting to watch the player he’s becoming — hopefully we can all play together. That would be good.
“He plays off the cuff. He’s imaginative. I think in this generation, they’re the things you need to be world class.”
Viewers will be able to support the National Emergencies Trust either through donating or sharing the campaign via dowhatyoucan.net. The National Emergencies Trust can also be found on Twitter, Facebook and Instagram