The Queen of Pop, 61, shared a video which incorrectly claimed that a coronavirus vaccine had already been created.
The clip showed a group called America’s Frontline Doctors, with Houston-based Dr Stella Immanuel claiming she had successfully treated over 350 Covid-19 patients with hydroxychloroquine.
Instagram soon placed a “false information” label on the video and directed users to a page which debunked the claims.
The post then disappeared from Madonna’s page – but not before many of the star’s 15 million followers had called her out.
According to the BBC, Annie Lennox was one of the people who criticised the star, commenting: “This is utter madness!!! I can’t believe that you are endorsing this dangerous quackery.
“Hopefully your site has been hacked and you’re just about to explain it.”
Prior to Madonna’s post, the clip had been shared widely on numerous social media networks with Twitter and Facebook both taking action to ban it earlier this week.
Donald Trump Jr is another famous face who shared it – earning himself a 12-hour ban from Twitter for doing so.
This is not the first time that Madonna has shared controversial coronavirus claims on Instagram.
In March, she faced a backlash after sharing a bizarre video of herself in the bath speaking directly to camera about the pandemic.
Calling the virus “the great equaliser,” she said: “What’s terrible about it is it’s made us equal in many ways.
“And what’s wonderful about it is that’s made us all equal in many ways.”
In a rather ominous final statement, she added: “Like I used to say at the end of human nature every night, if the ship goes down, we’re all going down together.”