Leaving in a hurry, she left a recording for Sonia Fowler (Natalie Cassidy) explaining her decision.
However, the end of her message led to fans believing she may tragically die off screen.
She told Sonia: “I shall miss you and I shall always love you as I know you love me. So, goodbye my dearest girl, your loving grandma Dot.”
Taking to Twitter to comment, one viewer shared: “Has Dot left EastEnders permanently? #EastEnders.”
Another wrote: “Dot will probably die when she’s in Ireland visiting Charlie. Her goodbye sounded so final. #Eastenders.”
EastEnders continues tonight on BBC One at 8pm.