Fifa has denied a request from Chelsea to freeze their one-year transfer ban while the club challenges the suspension.
Chelsea were banned from signing players in either of the next two transfer windows after breaching rules regarding the registration of minors.
Fifa’s appeal panel chairman, Thomas Bodstrom, has denied the club chance to continue working in the transfer market while they appeal the ban.
The decision means Chelsea would now have to take the issue to the Court of Arbitration for Sport in order to grant an interim ruling.
Fifa found the Stamford Bridge club guilty of breaching article 19 of the regulations in the case of 29 minor players, as well as a number of other infringements relating to requirements for registering players.
An additional fine of £460,000 has been given to the Londoners, who were also given three months to rectify the irregularities surrounding the minor players involved.
Chelsea believe they have grounds for appeal, however, after a full audit of over 100 transfers found just a few breaches that they feel do not constitute serious offences.